Novel bacteriophage lambda cloning vector.

Novel bacteriophage lambda mutation affecting lambda head assembly.

A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts. Both properties are due to dc10 since all phage revertants for one phenotype...

Genetic map of bacteriophage lambda.

Genetic recombination in bacteriophage lambda

© The Author 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Genetic recombination in bacteriop...

In vitro assembly of bacteriophage Lambda heads.

The assembly of plaque-forming particles in cell-free extracts of induced lambda lysogens was observed two ways. (i) DNA isolated from a lambda-related phage, 434 for example, is added to an extract of an induced lambda lysogen, and plaque-formers with the genotype of the added DNA are detected. (ii) One extract from an induced lambda lysogen that carries an amber mutation in one of the head ge...

On the Internal Structure of Bacteriophage Lambda

The structure of bacteriophage lambda has been studied by electron microscopy of negatively stained particles. The phage particles will eject their DNA if they are heated or dialyzed against a chelating agent. The ghost particles, so formed, have a channel running down their tails. Since the channel is not visible in normal particles, the channel may be filled with part of the DNA molecule. Up ...